The M&N constructed the approximately 0.8-mile Neenah Branch, running from Menasha, Wis., to Neenah, Wis., in 1881. By the mid-1880s, the Menasha, Appleton and Neenah Branches were collectively referred to as the Appleton Branch on timetables.
It was the Appleton Branch of the Superior Division of the Chicago, Milwaukee & St. Paul (CM&StP), or Milwaukee Road (MILW), after they took over the M&N in 1893. The MILW reorganized as the Chicago, Milwaukee, St. Paul & Pacific (CMStP&P) in 1928.
The Neenah Branch is still in use, currently operated by the Canadian National (CN).